Detailed view for Tb927.3.1540

Basic information

TDR Targets ID: 19450
Trypanosoma brucei, CAAX amino terminal protease, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6111 | Length (AA): 302 | MW (Da): 33833 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 5

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02517   CAAX protease self-immunity

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
119 161 2q80 (A) 114 150 51.00 0.49 0.15 0.309284 1.92
121 157 4mu9 (A) 261 297 32.00 0.65 0.1 0.388417 0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129169)

Species Accession Gene Product
Arabidopsis thaliana AT2G36305   CAAX prenyl protease 2
Brugia malayi Bm1_12350   CAAX amino terminal protease family protein
Candida albicans CaO19.11306   ras and a-factor terminal proteolysis
Candida albicans CaO19.3825   ras and a-factor terminal proteolysis
Caenorhabditis elegans CELE_F48F5.5   Protein FCE-2
Dictyostelium discoideum DDB_G0292570   hypothetical protein
Drosophila melanogaster Dmel_CG4852   severas
Echinococcus granulosus EgrG_000535900   CAAX prenyl protease 2
Entamoeba histolytica EHI_187200   CAAX prenyl protease family
Echinococcus multilocularis EmuJ_000535900   CAAX prenyl protease 2
Giardia lamblia GL50803_7029   Hypothetical protein
Homo sapiens ENSG00000173653   Ras converting CAAX endopeptidase 1
Leishmania braziliensis LbrM.26.2620   CAAX prenyl protease 2, putative,peptidase with unknown catalytic mechanism (family U48)
Leishmania donovani LdBPK_262720.1   CAAX prenyl protease 2, putative
Leishmania infantum LinJ.26.2720   CAAX prenyl protease 2, putative,peptidase with unknown catalytic mechanism (family U48)
Leishmania major LmjF.26.2690   CAAX prenyl protease 2, putative,peptidase with unknown catalytic mechanism (family U48)
Leishmania mexicana LmxM.26.2690   CAAX prenyl protease 2, putative,peptidase with unknown catalytic mechanism (family U48)
Loa Loa (eye worm) LOAG_09521   hypothetical protein
Mus musculus ENSMUSG00000024889   RCE1 homolog, prenyl protein peptidase (S. cerevisiae)
Oryza sativa 9268853   Os05g0357700
Saccharomyces cerevisiae YMR274C   Rce1p
Schistosoma japonicum Sjp_0201200   ko:K08658 prenyl protein peptidase, putative
Schistosoma japonicum Sjp_0308190   expressed protein
Schistosoma mansoni Smp_017650.2   family U48 unassigned peptidase (U48 family)
Schistosoma mansoni Smp_017650.1   family U48 unassigned peptidase (U48 family)
Trypanosoma brucei gambiense Tbg972.3.1300   CAAX prenyl protease 2, putative,peptidase with unknown catalytic mechanism (family U48), putative
Trypanosoma brucei Tb927.3.1540   CAAX amino terminal protease, putative
Trypanosoma congolense TcIL3000_0_31320   CAAX amino terminal protease, putative
Trypanosoma cruzi TcCLB.510421.29   CAAX prenyl protease 2, putative
Trypanosoma cruzi TcCLB.510089.39   peptidase with unknown catalytic mechanism (family U48)
Trichomonas vaginalis TVAG_226550   Clan U, family U48, CaaX prenyl peptidase 2-like

Essentiality

Tb927.3.1540 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1540 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1540 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.1540 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.1540 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Saccharomyces cerevisiae Rce1p Compounds References
Homo sapiens Ras converting CAAX endopeptidase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0096 0.2847 1
0.0107 0.4707 1
0.0305 0.5388 0.7046
0.0111 0.4363 1
0.0097 0.5176 0.5
0.0217 0.6361 1
0.01 0.383 1
0.0103 0.3088 0.2902
0.028 0.2894 1
0.0096 0.3541 0.5
0.0583 0.2747 0.2745
0.028 0.2902 1
0.0183 0.5758 1
0.0217 0.8965 1
0.0179 0.3125 1
0.0111 0.7333 1
0.0221 0.665 1
0.0184 0.3692 1
0.0177 0.5003 1
0.0221 0.5477 0.5
0.0221 0.7624 0.5
0.0185 0.457 1
0.0221 1 0.5
0.0095 0.506 1
0.0111 0.3078 1
0.02 0.4092 1
0.0278 0.5222 1
0.0206 0.2715 1
0.0111 0.4831 0.5
0.019 0.3846 1
0.022 0.2621 0.7428
0.0101 0.2647 1
0.0472 0.2792 1
0.019 0.5477 0.5
0.0179 0.6546 1
0.0199 0.3921 1
0.0374 0.4501 1
0.0211 0.2624 1
0.0109 1 1
0.0092 0.2672 1
0.0102 0.4935 1
0.028 0.2712 1
0.0094 0.2635 1
0.0182 0.258 1
0.0101 0.4356 1
0.0199 1 1
0.0181 1 1
0.019 0.3846 1
0.0221 0.6754 1
0.0097 0.8917 0
0.0221 1 0.5
0.0178 0.5666 1
0.0185 0.3128 0.5745
0.0097 0.2547 1
0.0095 1 0.5
0.0457 0.2754 1
0.038 0.2654 1
0.0183 0.3914 1
0.0276 0.4262 1
0.0095 0.2635 1
0.0083 0.3021 0.5
0.0474 0.5488 1
0.0091 0.2659 0.9632
0.0094 0.2977 1
0.0193 0.5746 1
0.0181 0.7444 1
0.0105 0.3591 1
0.0312 0.3744 1
0.0296 0.4029 1
0.0196 0.7608 1
0.0111 0.3491 0.5
0.0565 0.2989 1
0.0197 0.4027 1
0.0364 0.7156 1
0.0093 0.2615 0.8233
0.0196 0.436 1
0.0189 0.3553 0.5
0.0188 0.5241 1
0.0107 0.3482 1
0.0187 0.4105 1
0.0234 0.3409 1
0.0206 0.2511 0.9826
0.0219 0.3282 1
0.0111 1 0.5
0.0204 0.7017 1
0.0091 0.3542 0.9327
0.0183 0.4851 0.5
0.0179 0.9598 1
0.0189 0.4996 1
0.029 0.5573 0.9422
0.0182 0.667 1
0.0221 0.511 1
0.0203 0.3592 0.4875
0.0111 0.5756 0.5
0.0183 0.6674 1
0.0091 0.4935 1
0.0181 0.7409 1
0.0195 0.3371 1
0.0101 0.3934 1
0.0098 0.4467 1
0.0111 0.3566 1
0.0103 0.262 1
0.009 0.5756 0.5
0.0286 0.2942 1
0.0194 0.4047 1
0.0093 0.3319 0.5
0.0093 0.3449 1
0.0092 0.2633 1
0.0583 0.2747 0.2745
0.0211 0.2764 1
0.0185 1 1
0.0195 0.3371 1
0.0565 0.2989 1
0.0102 1 0.5
0.0374 0.4098 1
0.0095 0.5176 0.5
0.0448 0.7819 1
0.0093 0.2508 1
0.0221 1 0.5
0.0185 0.5769 1
0.0181 0.5389 0.5
0.0184 0.3893 1
0.0177 0.3338 1
0.0221 0.511 1
0.0221 1 1
0.0183 0.5191 1
0.0183 0.5863 1
0.046 1 0.5
0.0108 0.4707 1
0.009 0.251 1
0.0098 0.2607 0.776
0.0284 0.3275 0.5
0.0187 0.4142 1
0.0111 0.3614 1
0.0369 0.3482 1
0.0578 0.5493 1
0.0111 1 0.5
0.0111 0.3703 0.5
0.0279 0.5504 0.9412
0.0192 0.4211 1
0.0097 0.8917 0
0.0069 0.4136 0
0.0691 0.621 1
0.0208 0.5172 0.5
0.0193 1 0.5
0.0111 0.4707 1
0.0569 0.2987 1
0.0221 0.2545 1
0.0092 0.5514 1
0.0276 0.781 0.5
0.0208 0.4725 1
0.0188 0.4294 0.5
0.0371 0.2663 1
0.0293 1 1
0.0207 0.2632 1
0.0694 0.9811 1
0.0077 0.3021 0.5
0.0104 0.3507 0.5
0.0183 0.5326 1
0.0099 0.2635 1
0.0292 0.8201 0.5
0.0311 1 1
0.0107 0.4152 1
0.0217 0.7499 1
0.01 0.2764 1
0.0099 0.4935 1
0.0183 0.6188 1
0.0091 0.3633 0.9547
0.0203 0.3647 1
0.0197 1 1
0.0181 0.2612 1
0.0094 0.2977 1
0.0178 0.4628 0.4566
0.0221 1 0.5
0.0093 0.3307 1
0.0111 0.4376 1
0.0095 0.4555 0
0.0221 0.7322 0.5
0.0095 1 1
0.0206 0.2882 0.7333
0.0181 0.6714 1
0.0181 0.5322 1
0.0111 1 0.5
0.0184 0.4855 1
0.039 0.2643 1
0.0102 0.2717 1
0.018 0.7421 1
0.0178 0.3932 0.5
0.0221 0.375 1
0.0183 0.6188 1
0.0185 1 1
0.0172 0.498 1
0.009 0.3526 1
0.018 0.2525 1
0.0081 0.3703 1
0.0097 0.4836 1
0.0111 0.5 0.5
0.0094 0.2977 1
0.009 0.4944 1
0.0089 0.2506 0.2852
0.0178 0.2591 1
0.0188 0.6118 1
0.0111 0.7053 1
0.0111 0.7238 0.5
0.0095 1 0.5
0.0188 0.3208 0.5
0.0179 0.3125 1
0.0111 0.372 1
0.0291 0.4024 0.9632
0.0111 0.4862 0.5
0.0195 0.3371 1
0.0195 0.4422 0.9145
0.0385 0.4554 1
0.0196 0.3709 1
0.0089 0.2858 1
0.0179 0.2548 0.5
0.0201 0.6435 1
0.0092 0.2984 1
0.0205 0.2512 1
0.0201 0.5754 1
0.0111 1 0.5
0.0111 0.2781 0.2633
0.0111 0.2766 1
0.0091 0.2914 1
0.0374 0.4501 1
0.0096 0.4291 1
0.0293 0.5869 1
0.0221 1 1
0.0092 0.2633 1
0.0273 0.253 1
0.0217 0.5769 1
0.0205 0.2564 1
0.0102 0.2594 0.2523
0.0089 0.2816 1
0.0197 0.2613 1
0.0197 0.4822 1
0.0111 0.9654 0.5
0.0212 1 0.5
0.0056 0.7778 0.7632
0.0221 1 0.5
0.0221 0.556 1
0.0188 0.2642 1
0.0279 0.3233 1
0.0111 0.3746 0.5
0.0393 0.5461 1
0.0192 0.2657 1
0.028 0.5527 1
0.0276 0.3683 1
0.0182 1 1
0.0087 0.2771 1
0.0193 0.7594 1
0.018 0.3075 1
0.0029 1 1
0.0111 0.2635 1
0.0111 0.5756 0.5
0.0192 0.8108 1
0.0076 0.2517 1
0.0565 0.2989 1
0.0111 0.9704 1
0.0205 0.7322 0.5
0.0186 0.3038 1
0.0111 0.2937 1
0.0091 0.2867 1
0.0104 0.4935 1
0.019 0.3808 1
0.0136 1 0.5
0.0111 0.285 1
0.0162 0.2514 1
0.0185 0.74 1
0.0205 0.2651 1
0.021 0.2886 1
0.0184 0.426 0.5
0.0089 0.3551 0.9535
0.0179 0.5513 1
0.0187 0.3609 1
0.0111 0.2826 0.8728
0.0221 0.8878 1
0.0277 0.3744 1
0.0089 0.2516 1
0.0107 0.3483 0.4694
0.0192 0.5048 1
0.0188 0.4433 1
0.0173 1 0.5
0.0384 0.4721 1
0.0111 0.4831 1
0.029 0.3722 1
0.0089 0.3691 1
0.0102 0.2594 0.2523
0.0102 0.2717 1
0.0111 0.5756 0.5
0.0091 0.4935 1
0.0106 0.3542 1
0.009 0.3426 1
0.0093 0.3188 1
0.0383 0.2634 1
0.0189 0.4988 1
0.01 0.3406 1
0.0181 0.5465 1
0.01 0.499 1
0.0099 0.2692 1
0.0181 0.2612 1
0.022 0.8974 1
0.0276 0.37 1
0.0183 0.2501 1
0.019 1 0.5
0.0089 0.3691 1
0.019 0.4434 1
0.0092 0.2582 1
0.0207 0.2512 1
0.0461 0.2556 1
0.0279 0.3233 1
0.0221 0.4434 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.3.1540 (Trypanosoma brucei), CAAX amino terminal protease, putative
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